VISIKOL developed a suite of products which provide researchers with an unprecedented view of tissues for enhanced characterization. We currently sell several reagents and supporting materials through our divbio which allow researchers to label and image tissues in 3D for complete characterization. We see our products as transformational in that they allow researchers to shift the paradigm of bio-imaging from the traditional 2D histological approach to a 3D approach. Below you can see our product categories which include tissue clearing reagents and kits for whole animal tissues (Visikol HISTO), 3D cell culture models such as organoids, spheroids and microtissues (Visikol HISTO-M), developmental and reproductive toxicology studies (Visikol TOX) and plant biology (Visikol for Plant Biology).
Human DOT1L(DOT1 Like, Histone H3 Methyltransferase) ELISA Kit ELK7502
Specifications
| 48T / 96T |
Product info:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DOT1 Like, Histone H3 Methyltransferase(DOT1L). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DOT1 Like, Histone H3 Methyltransferase(DOT1L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DOT1 Like, Histone H3 Methyltransferase(DOT1L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DOT1 Like, Histone H3 Methyltransferase(DOT1L) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Enzyme & Kinase;
Species reactivity:
Human
Alternative names:
DOT1; KMT4; Histone-lysine N-methyltransferase, H3 lysine-79 specific; Histone H3-K79 methyltransferase; Lysine N-methyltransferase 4
Sample type:
Tissue homogenates, cell lysates and other biological fluids.
Detection range:
Sensitivity:
0.058 ng/mL
Standard:
10 ng/mL
Detection method:
Sandwich
Assay time:
3.5h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com