Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inositol Polyphosphate-5-Phosphatase 145kDa(INPP5D). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Inositol Polyphosphate-5-Phosphatase 145kDa(INPP5D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inositol Polyphosphate-5-Phosphatase 145kDa(INPP5D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inositol Polyphosphate-5-Phosphatase 145kDa(INPP5D) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Enzyme & Kinase;

Species reactivity:

Human

Alternative names:

SHIP; SHIP1; SIP-145; hp51CN; Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatase 1; SH2 domain-containing inositol 5'-phosphatase 1

Sample type:

Serum, plasma, tissue homogenates and other biological fluids

Detection range:

Sensitivity:

13.5 pg/mL

Standard:

2000 pg/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com