Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with Anti-Immunoglobulin A(Anti-IgA) protein. Standards or samples are added to the appropriate microtiter plate wells then with Anti-Immunoglobulin A(Anti-IgA) protein. Then add a biotin-conjugated Anti-Immunoglobulin A(Anti-IgA) protein. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anti-Immunoglobulin A Antibody(Anti-IgA) , biotin-conjugated protein and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Immunoglobulin A Antibody(Anti-IgA) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Infection immunity;Immune molecule;Hematology;Pulmonology;

Species reactivity:

Human

Alternative names:

IgA

Sample type:

serum, plasma and other biological fluids

Detection range:

Sensitivity:

2.36 ng/mL

Standard:

400 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com