Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HE4(Epididymal protein 4). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HE4(Epididymal protein 4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HE4(Epididymal protein 4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HE4(Epididymal protein 4) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Ovarian cancer

Species reactivity:

Human

Alternative names:

Epididymis Protein 4; dJ461P17.6 ; EDDM4 ; epididymal protein 4 ; Epididymal secretory protein E4 ; Epididymis specific whey acidic protein type four disulfide core ; HE 4 ; Major epididymis specific protein E4 ; Major epididymis-specific protein E4 ; MGC57529 ; Putative protease inhibitor WAP5 ; WAP 5 ; WAP domain containing protein HE4 ; WAP domain containing protein HE4 V4 ; WAP four disulfide core domain 2 ; WAP four disulfide core domain protein 2 ; WAP four-disulfide core domain protein 2 ; WAP5 ; WFDC 2 ; WFDC2 ; WFDC2_HUMAN

Sample type:

serum, plasma and other biological fluids

Detection range:

Sensitivity:

5.15 ng/mL

Standard:

1000 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com