Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B- and T-lymphocyte attenuator(BTLA). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to B- and T-lymphocyte attenuator(BTLA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B- and T-lymphocyte attenuator(BTLA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B- and T-lymphocyte attenuator(BTLA) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Cardiac function; Cancer

Species reactivity:

Human

Alternative names:

B and T lymphocyte associated protein; B and T lymphocyte attenuator; B and T lymphocyte associated; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA; BTLA_HUMAN; BTLA1; CD272; CD272 antigen; FLJ16065; MGC129743

Sample type:

Serum, plasma, urine, tissue homogenates, cell culture supernates and other biological fluids

Detection range:

Sensitivity:

0.22 ng/mL

Standard:

30 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com