Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PNX-20(Phoenixin-20). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PNX-20(Phoenixin-20). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PNX-20(Phoenixin-20), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PNX-20(Phoenixin-20) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;

Species reactivity:

Human

Alternative names:

Small integral membrane protein; Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 7 kDa; MITRAC7; Phoenixin-14; PNX-14; Phoenixin-20; PNX-20; SMIM20; C4orf52

Sample type:

Serum, plasma and other biological fluids.

Detection range:

Sensitivity:

7.4 pg/mL

Standard:

1000 pg/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com