Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GPR39( G protein coupled receptor 39). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GPR39( G protein coupled receptor 39). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GPR39( G protein coupled receptor 39), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GPR39( G protein coupled receptor 39) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Neuro science;

Species reactivity:

Human

Alternative names:

G protein coupled receptor GPR39; G Protein-Coupled Receptor 39; G-protein coupled receptor 39; GPR 39; Gpr39

Sample type:

Tissue homogenates and other biological fluids.

Detection range:

Sensitivity:

0.108 ng/mL

Standard:

20 ng/mL

Detection method:

Sandwich

Assay time:

4.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com