Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FoxN1(Forkhead box N1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to FoxN1(Forkhead box N1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FoxN1(Forkhead box N1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FoxN1(Forkhead box N1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Immunology

Species reactivity:

Human

Alternative names:

Forkhead box N1; FKHL20; Forkhead box protein N1; FOXN 1; FOXN1; Transcription factor winged-helix nude

Sample type:

serum, plasma, tissue homogenates, cell lysates, saliva, cell culture supernates and other biological fluids

Detection range:

Sensitivity:

6.8 pg/mL

Standard:

1000 pg/mL

Detection method:

Sandwich

Assay time:

4.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com