Product info:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gastric Intrinsic Factor(GIF) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gastric Intrinsic Factor(GIF). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gastric Intrinsic Factor(GIF) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Endocrinology;

Species reactivity:

Human

Alternative names:

TCN3; IF; IFMH; INF; Transcobalamin III; Vitamin B Synthesis

Sample type:

Serum, plasma and other biological fluids.

Detection range:

Sensitivity:

0.95 ng/mL

Standard:

200 ng/mL

Detection method:

Competitive Inhibition

Assay time:

2h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com