Product info:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Ferroportin(FPN) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ferroportin(FPN). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ferroportin(FPN) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Metabolic pathway;Infection immunity;Hematology;

Species reactivity:

Human

Alternative names:

SLC40A1; FPN1; HFE4; IREG1; MTP1; SLC11A3; Solute Carrier Family 40,Member 1; Iron-Regulated Transporter

Sample type:

tissue homogenates, cell lysates and other biological fluids

Detection range:

Sensitivity:

91.4 pg/mL

Standard:

20000 pg/mL

Detection method:

Competitive Inhibition

Assay time:

2h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com