Product info:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cyclic Guanosine Monophosphate(cGMP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cyclic Guanosine Monophosphate(cGMP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclic Guanosine Monophosphate(cGMP) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;Metabolic pathway;

Species reactivity:

General

Alternative names:

c-GMP; 3',5'-Cyclic GMP; Cyclic 3',5'-GMP; Guanosine 3',5'-Cyclic Phosphate

Sample type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection range:

Sensitivity:

45.2 pg/mL

Standard:

10000 pg/mL

Detection method:

Competitive Inhibition

Assay time:

2h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com