Product info:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Phosphatidylinositol Trisphosphate(PIP3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphatidylinositol Trisphosphate(PIP3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphatidylinositol Trisphosphate(PIP3) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;

Species reactivity:

General

Alternative names:

PtdIns(3,4,5)P3

Sample type:

serum, plasma and other biological fluids

Detection range:

Sensitivity:

2.83 ng/mL

Standard:

500 ng/mL

Detection method:

Competitive Inhibition

Assay time:

2h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com