Product info:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 6-keto-PGF1 alpha protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 6-keto-PGF1 alpha. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 6-keto-PGF1 alpha in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Neuro science;

Species reactivity:

Rat

Alternative names:

6-keto-PGF1 alpha; 6ketoPGF1α

Sample type:

Serum, plasma and other biological fluids.

Detection range:

Sensitivity:

1.4 pg/ml

Standard:

500 pg/ml

Detection method:

Competitive Inhibition

Assay time:

2h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com