Product info:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-Hydroxydeoxyguanosine(8-OHdG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-Hydroxydeoxyguanosine(8-OHdG). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-Hydroxydeoxyguanosine(8-OHdG) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology;

Species reactivity:

Human

Alternative names:

8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 8-Hydroxydeoxyguanosine

Sample type:

serum, plasma and other biological fluids

Detection range:

Sensitivity:

26.29 pg/mL

Standard:

6000 pg/mL

Detection method:

Competitive Inhibition

Assay time:

2h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com