Product info:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Indole 3 Acetic Acid(IAA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Indole 3 Acetic Acid(IAA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Indole 3 Acetic Acid(IAA) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;Hormone metabolism;

Species reactivity:

Plant

Alternative names:

IA-A; Indolylacetic Acid; Indoleacetic Acid; Heteroauxin

Sample type:

tissue or cell culture supernates

Detection range:

Sensitivity:

0.89 ng/mL

Standard:

200 ng/mL

Detection method:

Competitive Inhibition

Assay time:

2h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com