Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to STAB1(Stabilin-1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to STAB1(Stabilin-1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain STAB1(Stabilin-1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of STAB1(Stabilin-1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal Transduction; Epigenetics and Nuclear Signaling

Species reactivity:

Human

Alternative names:

STAB1 ; Stabilin-1 ; Fasciclin ; EGF-like ; laminin-type EGF-like and link domain-containing scavenger receptor 1 ; FEEL-1 ; MS-1 antigen ; FEEL1 ; KIAA0246 ; FEX1 ; FELE-1 ; STAB-1 ; CLEVER-1 ; common lymphatic endothelial and vascular endothelial receptor-1

Sample type:

serum, plasma, tissue homogenates, cell lysates and other biological fluids

Detection range:

0.16-10 ng/mL

Sensitivity:

0.056 ng/mL

Standard:

10 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com