Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to COL1A1(Pro-Collagen I alpha 1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to COL1A1(Pro-Collagen I alpha 1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL1A1(Pro-Collagen I alpha 1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of COL1A1(Pro-Collagen I alpha 1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal Transduction

Species reactivity:

Human

Alternative names:

Alpha 1 type I collagen; Alpha 2 type I collagen; alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; Alpha-1 type I collagen; alpha1(I) procollagen

Sample type:

serum, plasma and other biological fluids

Detection range:

Sensitivity:

15 pg/mL

Standard:

2500 pg/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com