Product info:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hemoglobin Beta(HBb) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hemoglobin Beta(HBb). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemoglobin Beta(HBb) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Hematology;

Species reactivity:

Human

Alternative names:

HB-B; HBD; CD113t-C; Beta Globin; Spinorphin

Sample type:

serum, plasma and erythrocyte lysates

Detection range:

7.82-500 pg/mL

Sensitivity:

2.94 pg/mL

Standard:

500 pg/mL

Detection method:

Competitive Inhibition

Assay time:

2h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com