Product info:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Krebs Von den Lungen-6(KL-6) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Krebs Von den Lungen-6(KL-6). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Krebs Von den Lungen-6(KL-6) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Interstitial lung disease

Species reactivity:

Human

Alternative names:

KL-6

Sample type:

serum, plasma, tissue homogenates and other biological fluids

Detection range:

12.5-800 IU/mL

Sensitivity:

5.9IU/mL

Standard:

800IU/mL

Detection method:

Competitive Inhibition

Assay time:

2h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com