Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like(BNIP3L). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like(BNIP3L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like(BNIP3L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like(BNIP3L) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Infection immunity;

Species reactivity:

Human

Alternative names:

BNIP3a; BNIP3H; BNIP3L; NIP3 like protein X; NIP3L; NIX; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like

Sample type:

Tissue homogenates, cell lysates and other biological fluids.

Detection range:

0.16-10 ng/mL

Sensitivity:

0.094 ng/mL

Standard:

10 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com