Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Branched-chain-amino-acid aminotransferase, mitochondrial(BCAT2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Branched-chain-amino-acid aminotransferase, mitochondrial(BCAT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Branched-chain-amino-acid aminotransferase, mitochondrial(BCAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Branched-chain-amino-acid aminotransferase, mitochondrial(BCAT2) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;Metabolic pathway

Species reactivity:

Mouse

Alternative names:

Branched-chain-amino-acid aminotransferase, mitochondrial; BCAT(m); Bcatm; Eca40; Bcat2

Sample type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection range:

15.63-1000 pg/mL

Sensitivity:

6.5 pg/mL

Standard:

1000 pg/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com