Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BALP(Bone-specific alkaline phosphatase). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to BALP(Bone-specific alkaline phosphatase). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BALP(Bone-specific alkaline phosphatase), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BALP(Bone-specific alkaline phosphatase) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Enzyme & Kinase;Metabolic pathway;Hepatology;Bone metabolism;

Species reactivity:

Dog

Alternative names:

BALP; BSAP; HOPS; AP-TNAP; TNSALP; Bone Alkaline Phosphatase; Alkaline Phosphatase,Tissue-Nonspecific Isozyme

Sample type:

serum, plasma and other biological fluids

Detection range:

0.32-20 ng/mL

Sensitivity:

0.116 ng/mL

Standard:

20 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com