At Solulink, we develop high-performance products that link biomolecules together. Our continuous efforts in new product development and service offerings enable you to remain at the forefront of advanced bioscience research. The Company’s proprietary HyNic/4FB chemistry is unique among bioconjugation methods in combining superb stability with high specificity and versatility, suitable for conjugation of all classes of biomolecules, including peptides, proteins, oligonucleotides, and carbohydrates, with all types of labels, including fluorophores, chromogenic moieties, surfaces, and small molecules. HyNic/4FB chemistry underlies the majority of Solulink’s extensive product catalog, comprised of various kits and tools that enable efficient and stable antibody, bead, and oligonucleotide labeling.
Dog CKB(Creatine Kinase, Brain) ELISA Kit ELK9543
Specifications
| 48T / 96T |
Product info:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Creatine Kinase, Brain(CKB). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Creatine Kinase, Brain(CKB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Creatine Kinase, Brain(CKB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Creatine Kinase, Brain(CKB) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Enzyme & Kinase;Metabolic pathway;Cardiovascular biology;Neuro science;
Species reactivity:
Dog
Alternative names:
CK-BB; CKBB; CK-B; CK-1
Sample type:
Tissue homogenates, cell lysates and other biological fluids.
Detection range:
0.79-50 ng/mL
Sensitivity:
0.32 ng/mL
Standard:
50 ng/mL
Detection method:
Sandwich
Assay time:
3.5h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com