Product info:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyroid Stimulating Hormone Receptor Antiboby(TSHRAb) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroid Stimulating Hormone Receptor Antiboby(TSHRAb). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroid Stimulating Hormone Receptor Antiboby(TSHRAb) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal Transduction; Neuroscience

Species reactivity:

Human

Alternative names:

TSHRAb

Sample type:

Serum, plasma and other biological fluids

Detection range:

1.57-100mIU/mL

Sensitivity:

0.85 mIU/mL

Standard:

100mIU/mL

Detection method:

Competitive Inhibition

Assay time:

2h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com