Product info:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-Hydroxynonenal(4-HNE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-Hydroxynonenal(4-HNE). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-Hydroxynonenal(4-HNE) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;

Species reactivity:

General

Alternative names:

4-HNE, HNE, 4-hydroxy-2-nonenal, 4-Hydroxynonenal

Sample type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection range:

0.63-40 ng/mL

Sensitivity:

0.38 ng/mL

Standard:

40 ng/mL

Detection method:

Competitive Inhibition

Assay time:

2h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com