Background:

Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. VSV-G protein is secreted into the culture medium as sendimentable vesicles from cells transfected with a VSV-G expression plasmid in the absence of other viral components. The VSV-G vesicles in the conditioned medium can be partially purified by pelleting through sucrose cushion ultracentrifugation.

Alternative names:

N/A

Clonality:

Monoclonal

Species reactivity:

N/A

Source:

Mouse

Species:

N/A

Cellular localization:

N/A

Isotype:

IgG1

Immunogen:

Synthetic Peptide

Concentration:

1mg/ml

Observed band:

N/A

Applications:

WB

Recommended dilution:

WB 1:5,000 IP 1:200 IF 1:1,000

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

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