Background:

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

Alternative names:

DDDDK epitope tag, DYKDDDDK epitope tag, ECS epitope tag

Clonality:

Monoclonal

Species reactivity:

N/A

Source:

Mouse

Species:

N/A

Cellular localization:

N/A

Isotype:

IgG1

Immunogen:

Synthetic Peptide

Concentration:

1mg/ml

Observed band:

N/A

Applications:

WB

Recommended dilution:

WB 1:5,000-10,000

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

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