Background:

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.

Alternative names:

N/A

Clonality:

Monoclonal

Species reactivity:

Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit,Sheep, Insect, Yeast

Source:

Mouse

Species:

Human

Cellular localization:

Cytoplasm,Cytoskeleton,Microtubule

Isotype:

IgG1

Immunogen:

Synthetic Peptide

Concentration:

1mg/ml

Observed band:

55kDa

Applications:

WB, IHC, IF

Recommended dilution:

WB 1:5,000 IHC 1:200 IF 1:100-200

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com