Background:

Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks. Knocking down intracellular PARP1 levels with siRNA or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks. In the absence of PARP1, when these breaks are encountered during DNA replication, the replication fork stalls, and double-strand DNA (dsDNA) breaks accumulate.

Alternative names:

PARP-1, Poly(ADP ribose) polymerase 1, sPARP1,ADPRT1, ADP ribosyltransferase NAD(+)

Clonality:

Monoclonal

Species reactivity:

Human

Source:

Mouse

Species:

Human

Cellular localization:

Nucleus

Isotype:

IgG1

Immunogen:

Synthetic Peptide

Concentration:

1mg/ml

Observed band:

116kDa

Applications:

WB, IHC

Recommended dilution:

WB 1:1,000-3,000   IHC 1:200-500

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com