TRIpure Total RNA Extraction Reagent is a reagent for total RNA extraction from various animal and plant, bacterial tissues/cells. It has strong cleavage ability to lyse cell and tissue samples in a short time and keep RNA intact in the sample. and effectively inhibit the degradation of RNA. The sample can be fully cleaved in the reagent. After centrifugation with chloroform, the solution forms a supernatant layer, an intermediate layer and an organic layer (lower red layer). The RNA is distributed in the upper aqueous phase, and the supernatant layer is collected. Total RNA can be recovered by precipitation of propanol. The extracted total RNA is high in purity and contains no protein or genomic DNA. It can be directly used for Northern, dot hybridization, mRNA purification, in vitro translation, RT-PCR, poly(A)+ selection, RNase protection analysis, and construction of cDNA libraries. A variety of molecular biology experiments.