Background:

Mouse monoclonal antibody to Cytokeratin 8

Alternative names:

CYK8; Keratin type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8

Clonality:

Monoclonal

Species reactivity:

H

Host:

Mouse

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within human Cytokeratin 8. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Applications:

WB, IH

Recommended dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/300)

Storage:

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB description:

Western blot analysis of Cytokeratin 8 expression in HepG2 (A) whole cell lysates.

IHC description:

Immunohistochemical analysis of Cytokeratin 8 staining in human gastric adenocarcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

Immunohistochemical analysis of Cytokeratin 8 staining in human hepatocellular carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IP description:

Immunohistochemical analysis of Cytokeratin 8 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com