Background:

Mouse monoclonal antibody to MYOD1

Alternative names:

BHLHC1; MYF3; MYOD; Myoblast determination protein 1; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3

Clonality:

Monoclonal

Species reactivity:

H

Host:

Mouse

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within human MYOD1. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Mouse IgG. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Applications:

WB, IH, IF

Recommended dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/300), IF (1/100 - 1/500)

Storage:

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB description:

Western blot analysis of MLH1 expression in Hela (A), U2OS (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of MYOD1 staining in human rhabdomyosarcoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com