Background:

Rabbit polyclonal antibody to FOXO1/3 (pS322+S325/pS318+S321)

Alternative names:

FOXO1; FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FOXO3; FKHRL1; FOXO3A; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1

Clonality:

Polyclonal

Species reactivity:

H, M, R, B, P

Host:

Rabbit

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FOXO1/3 (pS322+S325/pS318+S321). The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IH

Recommended dilution:

WB (1/500 - 1/1000), IH (1/50 - 1/200)

Storage:

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB description:

Western blot analysis of FOXO1/3 (pS322+S325/pS318+S321) expression in Beas2B (A), A549 (B), HepG2 (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of FOXO1/3 (pS322+S325/pS318+S321) staining in human thyroid gland formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

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