Background:

Mouse monoclonal antibody to His-tag

Alternative names:

Clonality:

Monoclonal

Species reactivity:

Host:

Mouse

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence of His-tag. The exact sequence is proprietary.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IF/IC, IP

Recommended dilution:

WB (1/2000 - 1/5000), IF/IC (1/200 - 1/500), IP (1/100 - 1/200)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of over-expressed Myc-tagged protein in 293T cell lysate.

IHC description:

N/A

IF/ICC description:

Immunofluorescent analysis of Myc-tag staining in 293T cells transfected with a Myc-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

Immunoprecipitation of Myc-tagged protein from HEK293T cells transfected with vector overexpressing Myc tag, using anti-Myc-tag Antibody.

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com