Background:

Rabbit polyclonal antibody to ACTN1/2/3/4

Alternative names:

ACTN1; Alpha-actinin-1; Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1; ACTN2; Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN3; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein; ACTN4; Alpha-actinin-4; F-actin cross-linking protein; Non-muscle alpha-actinin 4

Clonality:

Polyclonal

Species reactivity:

H, M, R, B, C, Z

Host:

Rabbit

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ACTN1/2/3/4. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IHC, IP

Recommended dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of ACTN1/2/3/4 expression in HeLa (A), HuvEc (B), mouse muscle (C), PC12 (D) whole cell lysates.

IHC description:

Immunohistochemical analysis of ACTN1/2/3/4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

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