Background:

Rabbit polyclonal antibody to 14-3-3 zeta (pT232)

Alternative names:

14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1

Clonality:

Polyclonal

Species reactivity:

H, M, R, B, C, P, S

Host:

Rabbit

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human 14-3-3 zeta. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IHC

Recommended dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of 14-3-3 zeta (pT232) expression in A431 UV-treated (A), K562 UV-treated (B), NIH3T3 UV-treated (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of 14-3-3 zeta (pT232) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

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