Background:

Rabbit polyclonal antibody to Carbonic Anhydrase 8

Alternative names:

CALS; Carbonic anhydrase-related protein; CARP; Carbonic anhydrase VIII; CA-VIII

Clonality:

Polyclonal

Species reactivity:

H, M, R, B, Mk

Host:

Rabbit

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Carbonic Anhydrase 8. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IHC

Recommended dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of Carbonic Anhydrase 8 expression in MCF7 (A), NS-1 (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of Carbonic Anhydrase 8 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

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