Background:

Rabbit polyclonal antibody to PKA C beta

Alternative names:

cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta

Clonality:

Polyclonal

Species reactivity:

H, M, R, B, C, P

Host:

Rabbit

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PKA C beta. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IHC

Recommended dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of PKA C beta expression in HEK293T (A), NIH3T3 (B), mouse lung (C), rat lung (D) whole cell lysates.

IHC description:

Immunohistochemical analysis of PKA C beta staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com