Background:

Rabbit polyclonal antibody to Histone H3

Alternative names:

H3FT; Histone H3.1t; H3/t; H3t; H3/g

Clonality:

Polyclonal

Species reactivity:

H, M, R

Host:

Rabbit

Immunogen:

KLH-conjugated synthetic peptide of human Histone H3

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IHC, IP, ChIP

Recommended dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/200), IP (1/50 - 1/100), ChIP (1/100 - 1/500)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of Histone H3 expression in A431 (A), Hela (B), mouse liver (C), rat kidney (D) whole cell lysates.

IHC description:

Immunohistochemical analysis of Histone H3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com