Background:

Rabbit polyclonal antibody to HuC

Alternative names:

HUC; PLE21; ELAV-like protein 3; Hu-antigen C; HuC; Paraneoplastic cerebellar degeneration-associated antigen; Paraneoplastic limbic encephalitis antigen 21

Clonality:

Polyclonal

Species reactivity:

H, M, R

Host:

Rabbit

Immunogen:

KLH-conjugated synthetic peptide of human HuC

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IHC, IP

Recommended dilution:

WB (1/500 - 1/1000), IH (1/50 - 1/200), IP (1/20 - 1/50)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of HuC expression in mouse brain (A), rat brain (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of HuC staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

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