Background:

Rabbit polyclonal antibody to eIF4A1

Alternative names:

DDX2A; EIF4A; Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1

Clonality:

Polyclonal

Species reactivity:

H, M, R

Host:

Rabbit

Immunogen:

Recombinant full length protein of human eIF4A1

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IF/IC, IP

Recommended dilution:

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200), IP (1/20 - 1/50)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of eIF4A1 expression in Hela (A), Jurkat (B), NIH3T3 (C), PC12 (D), mouse liver (E) whole cell lysates.

IHC description:

N/A

IF/ICC description:

Immunofluorescent analysis of eIF4A1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

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