Background:

Rabbit polyclonal antibody to p47 phox (pS370)

Alternative names:

NOXO2; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox

Clonality:

Polyclonal

Species reactivity:

H, M, R, Rb

Host:

Rabbit

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human p47 phox. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IF/IC, IP

Recommended dilution:

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of p47 phox (pS370) expression in HeLa serum starvation-treated (A), THP1 (B), Raw264.7 serum starvation-treated (C), H9C2 serum starvation-treated (D) whole cell lysates.

IHC description:

N/A

IF/ICC description:

Immunofluorescent analysis of p47 phox (pS370) staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

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