Background:

Rabbit polyclonal antibody to LDHB

Alternative names:

L-lactate dehydrogenase B chain; LDH-B; LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46

Clonality:

Polyclonal

Species reactivity:

H, M, R

Host:

Rabbit

Immunogen:

Recombinant full length protein of human LDHB

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IHC

Recommended dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of LDHB expression in A431 (A), Jurkat (B), A549 (C), mouse kidney (D), mouse lung (E), rat brain (F) whole cell lysates.

IHC description:

Immunohistochemical analysis of LDHB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com