Background:

Rabbit polyclonal antibody to HADH

Alternative names:

HAD; HADHSC; SCHAD; Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase

Clonality:

Polyclonal

Species reactivity:

H, M, R

Host:

Rabbit

Immunogen:

Recombinant full length protein of human HADH

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IHC

Recommended dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of HADH expression in Jurkat (A), HT29 (B), HL60 (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of HADH staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com