Background:

Rabbit polyclonal antibody to GAP43

Alternative names:

Neuromodulin; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46

Clonality:

Polyclonal

Species reactivity:

H, M, R

Host:

Rabbit

Immunogen:

Recombinant protein corresponding to human GAP43.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IHC

Recommended dilution:

WB (1/1000 - 1/3000), IH (1/500 - 1/1000)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of GAP43 expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of GAP43 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

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