Background:

Mouse monoclonal antibody to Cytokeratin 18

Alternative names:

CYK18; Keratin type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18

Clonality:

Monoclonal

Species reactivity:

H, M, R

Host:

Mouse

Immunogen:

Recombinant protein corresponding to human Cytokeratin 18.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IF/IC

Recommended dilution:

WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of Cytokeratin 18 expression in HepG2 (A), Hela (B), mouse liver (C), mouse skeletal muscle (D), C2C12 (E), rat heart (F) whole cell lysates.

IHC description:

N/A

IF/ICC description:

Immunofluorescent analysis of Cytokeratin 18 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com