Background:

Rabbit polyclonal antibody to TRAF3IP3

Alternative names:

T3JAM; TRAF3-interacting JNK-activating modulator; TRAF3-interacting protein 3

Clonality:

Polyclonal

Species reactivity:

H, B

Host:

Rabbit

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TRAF3IP3. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IHC, IP

Recommended dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of TRAF3IP3 expression in MDAMB453 (A), Jurkat (B), HeLa (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of TRAF3IP3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

Immunoprecipitation of TRAF3IP3 from 0.5mg Mouse cortex tissue lysate, using 5ug of anti-TRAF3IP3 Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse cortex extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with anti-TRAF3IP3 Antibody.

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

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