Background:

Rabbit polyclonal antibody to SHBG

Alternative names:

Sex hormone-binding globulin; SHBG; Sex steroid-binding protein; SBP; Testis-specific androgen-binding protein; ABP; Testosterone-estradiol-binding globulin; TeBG; Testosterone-estrogen-binding globulin

Clonality:

Polyclonal

Species reactivity:

H, M

Host:

Rabbit

Immunogen:

Recombinant full length protein of human SHBG

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IHC

Recommended dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of SHBG expression in A549 (A), mouse testis (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of SHBG staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

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