Background:

Rabbit polyclonal antibody to BRK (pY447)

Alternative names:

BRK; Protein-tyrosine kinase 6; Breast tumor kinase; Tyrosine-protein kinase BRK

Clonality:

Polyclonal

Species reactivity:

H, R

Host:

Rabbit

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human BRK. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IF/IC

Recommended dilution:

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of BRK (pY447) expression in HEK293T (A), rat kidney (B), rat lung (C) whole cell lysates.

IHC description:

N/A

IF/ICC description:

Immunofluorescent analysis of BRK (pY447) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com