Background:

Rabbit polyclonal antibody to RFC5

Alternative names:

Replication factor C subunit 5; Activator 1 36 kDa subunit; A1 36 kDa subunit; Activator 1 subunit 5; Replication factor C 36 kDa subunit; RF-C 36 kDa subunit; RFC36

Clonality:

Polyclonal

Species reactivity:

H

Host:

Rabbit

Immunogen:

Recombinant full length protein of human RFC5

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IHC

Recommended dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/100)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of RFC5 expression in SKOV3 (A), MCF7 (B), mouse liver (C), mouse spleen (D) whole cell lysates.

IHC description:

Immunohistochemical analysis of RFC5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

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